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Product Info Summary
| SKU: | PA2141-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Collagen II/COL2A1 Antibody
View all Collagen II Antibodies
SKU/Catalog Number
PA2141-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen II/COL2A1 Antibody catalog # PA2141-1. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen II/COL2A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2141-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2141-1 is reactive to COL2A1 in Mouse, Rat
Applications
PA2141-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
170 kDa
Calculated molecular weight
141.785kDa
Background of Collagen II
Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Mouse
Validation Images & Assay Conditions
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Anti-Collagen II antibody, PA2141-1, IHC(F)
IHC(F): Rat Trachea Tissue
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Anti-Collagen II antibody, PA2141-1, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Protein Target Info & Infographic
Gene/Protein Information For COL2A1 (Source: Uniprot.org, NCBI)
Gene Name
COL2A1
Full Name
Collagen alpha-1(II) chain
Weight
141.785kDa
Superfamily
fibrillar collagen family
Alternative Names
Alpha-1 type II collagen; ANFH; AOM; cartilage collagen; Chondrocalcin; COL11A3; COL2A1; Collagen 2; collagen alpha-1(II) chain; Collagen II; collagen II, alpha-1 polypeptide; collagen type 2; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital); collagen, type II, alpha 1; Collagen-2; MGC131516; SEDC COL2A1 ANFH, AOM, COL11A3, SEDC, STL1 collagen type II alpha 1 chain collagen alpha-1(II) chain|alpha-1 type II collagen|arthroophthalmopathy, progressive (Stickler syndrome)|cartilage collagen|chondrocalcin|collagen II, alpha-1 polypeptide|collagen, type II, alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL2A1, check out the COL2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen II/COL2A1 Antibody (PA2141-1)
Hello CJ!
PA2141-1 has been cited in 42 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang R,Zhang Q,Zou Z,Li Z,Jin M,An J,Li H,Ma J.Curcumin Supplementation Enhances Bone Marrow Mesenchymal Stem Cells to Promote the Anabolism of Articular Chondrocytes and Cartilage Repair.Cell Transplant.2021 Jan-Dec;30:963689721993776.doi:10.1177/096368
Species: Rat
PA2141-1 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:1:200
Guo Z,Qiu C,Mecca C,Zhang Y,Bian J,Wang Y,Wu X,Wang T,Su W,Li X,Zhang W,Chen B,Xiang H.Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration.BMC Musculoskelet Disord.2021 Jan 13;22(1):77.doi:10.1186/s12891-020-03934-7.PMID:3344
Species: Human,Rat
PA2141-1 usage in article: APP:IF, SAMPLE:NP CELL, DILUTION:1:100
Zhang P,Chang BG.Inhibitory effect on the apoptosis of disc nucleus pulposus cells in rats by silencing COL9A3 gene to mediate MAPK signaling pathway: a study on the function and mechanism.Eur Rev Med Pharmacol Sci.2020 Sep;24(17):8653-8664.doi:10.26355/e
Species: Rat
PA2141-1 usage in article: APP:IHC, SAMPLE:NUCLEUS PULPOSUS CELL, DILUTION:1:200
Lan Q,Lu R,Chen H,Pang Y, Xiong F,Shen C,Qin Z,Zheng L,Xu G,Zhao J.MMP-13 enzyme and pH responsive theranostic nanoplatform for osteoarthritis.J Nanobiotechnology.2020 Aug 27;18(1):117.doi:10.1186/s12951-020-00666-7. PMID:32854712;PMCID:PMC7450974.
Species: Mouse
PA2141-1 usage in article: APP:IF, SAMPLE:CHONDROCYTES, DILUTION:1:200
Sun M,Lu Z,Cai P,Zheng L,Zhao J.Salidroside enhances proliferation and maintains phenotype of articular chondrocytes for autologous chondrocyte implantation (ACI) via TGF-β/Smad3 Signal.Biomed Pharmacother.2020 Feb;122:109388.doi:10.1016/j.biopha.2019.109
Species: Rat
PA2141-1 usage in article: APP:IF, SAMPLE:CHONDROCYTES, DILUTION:1:200
Ni B,Huang X,Xi Y,Mao Z,Chu X,Zhang R,Ma X,You H.Neferine Inhibits Expression of Inflammatory Mediators and Matrix Degrading Enzymes in IL-1β-Treated Rat Chondrocytes via Suppressing MAPK and NF-κB Signaling Pathways.Inflammation.2020 Aug;43(4):1209-1221.
Species: Rat
PA2141-1 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA
Onder S,Bilgili SG,Bulut G,Celik HT,Oguztuzun S,Onder H,Calka O,Karadag AS.Biochemical and histopathologic assessment of effects of acitretin on epiphyseal growth plate in rats.Postepy Dermatol Alergol.2020 Jun;37(3):346-352.doi:10.5114/ada.2020.95983.Epu
Species: Rat
PA2141-1 usage in article: APP:IHC, SAMPLE:PROXIMAL TIBIA, DILUTION:NA
Zhang B,Wang C,Zhang Y,Jiang Y,Qin Y,Pang D,Zhang G,Liu H,Xie Z,Yuan H,Ouyang H,Wang J,Tang X.A CRISPR-engineered swine model of COL2A1 deficiency recapitulates altered early skeletal developmental defects in humans.Bone.2020 Aug;137:115450.doi:10.1016/j.
Species: Pig
PA2141-1 usage in article: APP:WB, SAMPLE:CARTILAGE AND TRACHEA, DILU TION:NA
Effects of Angelicae Pubescentis and Loranthi Decotion on repairing knee joint cartilages in rats
Microfluidics-based optimization of neuroleukin-mediated regulation of articular chondrocyte proliferation
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Collagen II/COL2A1 Antibody
Question
I am looking for using your anti-Collagen II/COL2A1 antibody for r-mmu-198933; immunoregulatory interactions between a lymphoid and a non-lymphoid cell studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
I appreciate your inquiry. This PA2141-1 anti-Collagen II/COL2A1 antibody is tested on rat trachea tissue, tissue lysate, heart tissue, brain tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-15
Question
I see that the anti-Collagen II/COL2A1 antibody PA2141-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-18
Question
I am interested in to test anti-Collagen II/COL2A1 antibody PA2141-1 on mouse tibia for research purposes, then I may be interested in using anti-Collagen II/COL2A1 antibody PA2141-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The products we sell, including anti-Collagen II/COL2A1 antibody PA2141-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-27
Question
Our team were satisfied with the WB result of your anti-Collagen II/COL2A1 antibody. However we have been able to see positive staining in fetal sternum extracellular using this antibody. Is that expected? Could you tell me where is COL2A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
Based on literature, fetal sternum does express COL2A1. Generally COL2A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183
Boster Scientific Support
Answered: 2019-11-01
Question
Is this PA2141-1 anti-Collagen II/COL2A1 antibody reactive to the isotypes of COL2A1?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The immunogen of PA2141-1 anti-Collagen II/COL2A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-08
Question
I have a question about product PA2141-1, anti-Collagen II/COL2A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2141-1 anti-Collagen II/COL2A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-29
Question
Is there a BSA free version of anti-Collagen II/COL2A1 antibody PA2141-1 available?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen II/COL2A1 antibody PA2141-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-08
Question
We have tried in the past anti-Collagen II/COL2A1 antibody for WB on blood last year. I am using rat, and We intend to use the antibody for IHC next. I am interested in examining blood as well as tibia in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
I took a look at the website and datasheets of our anti-Collagen II/COL2A1 antibody and it seems that PA2141-1 has been tested on rat in both WB and IHC. Thus PA2141-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibia using anti-Collagen II/COL2A1 antibody PA2141-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-02
Question
Would anti-Collagen II/COL2A1 antibody PA2141-1 work for IHC with tibia?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
According to the expression profile of tibia, COL2A1 is highly expressed in tibia. So, it is likely that anti-Collagen II/COL2A1 antibody PA2141-1 will work for IHC with tibia.
Boster Scientific Support
Answered: 2018-05-24
Question
Will PA2141-1 anti-Collagen II/COL2A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
It shows on the product datasheet, PA2141-1 anti-Collagen II/COL2A1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-29
Question
I have attached the WB image, lot number and protocol we used for tibia using anti-Collagen II/COL2A1 antibody PA2141-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-29
Question
I was wanting to use your anti-Collagen II/COL2A1 antibody for IHC for mouse tibia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse tibia identification?
B. Jones
Verified customer
Asked: 2017-12-26
Answer
As indicated on the product datasheet, PA2141-1 anti-Collagen II/COL2A1 antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse tibia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-26
Question
Is a blocking peptide available for product anti-Collagen II/COL2A1 antibody (PA2141-1)?
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
We do provide the blocking peptide for product anti-Collagen II/COL2A1 antibody (PA2141-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-14
Question
We are currently using anti-Collagen II/COL2A1 antibody PA2141-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
The anti-Collagen II/COL2A1 antibody (PA2141-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-06
Question
We have seen staining in mouse blood. Any tips? Is anti-Collagen II/COL2A1 antibody supposed to stain blood positively?
D. Moore
Verified customer
Asked: 2016-08-19
Answer
From what I have seen in literature blood does express COL2A1. From what I have seen in Uniprot.org, COL2A1 is expressed in tibia, blood, embryonic stem cell muscle, fetal sternum, among other tissues. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183
Boster Scientific Support
Answered: 2016-08-19
